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Built in 1883, Columbia Firehouse now stands as a historic, beautifully appointed American brasserie and barroom in the heart of Alexandria’s Old Town. The sumptuous dining rooms, atrium and outdoor patio are devoted to familiar comfort foods, oysters and shellfish, mussels, small plates for sharing, salads, steaks and nightly suppers. The handsome barroom features regional craft beers, stylish wines and classic cocktails. Open for lunch, dinner and weekend brunch, Columbia Firehouse is an essential neighborhood gathering place for friends and family, and an altogether timeless classic.

LUNCH
DINNER • RAW BAR
BRUNCH
DESSERT
BARROOM
CATERING
COCKTAILS
WINE AND BEER
OUR TEAM

Built in 1883, Columbia Firehouse now stands as a historic, beautifully appointed American brasserie and barroom in the heart of Alexandria’s Old Town. The sumptuous dining rooms, atrium and outdoor patio are devoted to familiar comfort foods, oysters and shellfish, mussels, small plates for sharing, salads, steaks and nightly suppers. The handsome barroom features regional craft beers, stylish wines and classic cocktails. Open for lunch, dinner and weekend brunch, Columbia Firehouse is an essential neighborhood gathering place for friends and family, and an altogether timeless classic.

In 1871, the City of Alexandria introduced the Columbia Steam Engine Fire Company. The outfit initially occupied Star Firehouse on the east side of the 100 block of South St. Asaph Street until 1883, when a new station was built for Columbia just across the street at 109 South St. Asaph. 127 years later, this beautifully preserved property was relaunched as Columbia Firehouse, a lively and sumptuous modern American brasserie located in the heart of Alexandria’s historic Old Town.

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LUNCH TUESDAY • FRIDAY 1130 • 3PM DINNER MONDAY 530 • 9PM TUESDAY • THURSDAY 530 • 10PM FRIDAY AND SATURDAY 530 • 11PM SUNDAY 430 • 9PM
BRUNCH SATURDAY AND SUNDAY 11 • 3PM BARROOM MONDAY 4PM • 130 AM TUESDAY • FRIDAY 1130AM • 130AM SATURDAY AND SUNDAY 11AM • 130AM LATE NIGHT BAR MENU AVAILABLE

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PRIVATE PARTIES

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ARCADIAFOOD.ORG

OUR HOURS

LUNCH

TUESDAY • FRIDAY 1130 • 3PM

DINNER

MONDAY 530 • 9PM TUESDAY • THURSDAY 530 • 10PM FRIDAY AND SATURDAY 530 • 11PM SUNDAY 430 • 9PM

BRUNCH

SATURDAY AND SUNDAY 11 • 3PM

BARROOM

MONDAY 4PM • 130 AM TUESDAY • FRIDAY 1130AM • 130AM SATURDAY AND SUNDAY 11AM • 130AM LATE NIGHT BAR MENU AVAILABLE

109 ST ASAPH STREET,ALEXANDRIA VA 22314 TELEPHONE 703 683 1776 / GOOGLE MAP

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Struecker, Benjamin; Butter, Antje; Hillebrandt, Karl; Polenz, Dietrich; Reutzel-Selke, Anja; Tang, Peter; Lippert, Steffen; Leder, Anne; Rohn, Susanne; Geisel, Dominik; Denecke, Timm; Aliyev, Khalid; Jöhrens, Korinna; Raschzok, Nathanael; Neuhaus, Peter; Pratschke, Johann; Sauer, Igor M

One approach of regenerative medicine to generate functional hepatic tissue in vitro is decellularization and recellularization, and several protocols for the decellularization of livers of different species have been published. This appears to be the first report on rat liver decellularization by perfusion under oscillating pressure conditions, intending to optimize microperfusion and minimize damage to the ECM. Four decellularization protocols were compared: perfusion via the portal vein (PV) or the hepatic artery (HA), with (+P) or without (-P) oscillating pressure conditions. All rat livers (n = 24) were perfused with 1% Triton X-100 and 1% sodium dodecyl sulphate, each for 90 min with a perfusion rate of 5 ml/min. Perfusion decellularization was observed macroscopically and the decellularized liver matrices were analysed by histology and biochemical analyses (e.g. levels of DNA, glycosaminoglycans and hepatocyte growth factor). Livers decellularized via the hepatic artery and under oscillating pressure showed a more homogeneous decellularization and less remaining DNA, compared with the livers of the other experimental groups. The novel decellularization method described is effective, quick (3 h) and gentle to the extracellular matrix and thus represents an improvement of existing methodology. Copyright © 2014 John Wiley Sons, Ltd. Copyright © 2014 John Wiley Sons, Ltd.

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Dawson, J; Knowles, R G; Pogson, C I

1992-03-03

We have developed a simple and sensitive method for the study of the relative rates of glucuronidation of compounds, in isolated liver cells, based on the incorporation of 14C from fructose into glucuronide conjugates. Liver cells from fasted rats are used to minimize any reduction of the specific activity by glycogenolysis. Although rates of glucuronidation are lower in isolated liver cells from fasted rats than in those from fed rats , because of a reduction in the concentration of UDP-glucuronic acid, it is possible to compare the rates of glucuronidation of different compounds. Radiolabelled glucuronides are separated from [14C]fructose and [14C]glucose, produced by the liver cells, by normal-phase HPLC on a polar amino-cyano column. The specific activity of the glucuronide was found to be approximately 50% of that of the [14C]fructose. Absolute amounts of glucuronide can be determined by measuring the specific activity of the [14C]glucose, also produced by liver cells from fructose, which reflects that of the glucose-6-phosphate and hence the UDP-glucuronic acid used for glucuronidation, although for the measurement of relative rates this would not be necessary. We have used this method to examine the kinetics of the glucuronidation of N-acetyl-p-aminophenol (acetaminophen), 4-nitrophenol and 1-naphthol in isolated rat liver cells. The method should be applicable to the study of the rates of glucuronidation of a range of aglycones and, unlike other methods, does not require glucuronide standards or radiolabelled aglycone.

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The Workshops provide leading experts and early career scientists from a diverse range of scientific backgrounds with a stimulating environment for the cross-fertilisation of interdisciplinary ideas. The programmes are carefully developed and are intended to champion the novel techniques and innovations that will underpin important scientific advances.

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Organisers: Frederic Berger and Ines Drinnenberg

Date: 4 – 7 November 2018

Reconstitution of cell cytoskeleton

Organisers: Manuel Théry and Laurent Blanchoin

Date: 27 – 30 January 2019

Chromatin-based regulation of development

Organisers: Benoit Bruneau and Joanna Wysocka

Date: 14 – 17 April 2019

New frontiers in the brain: unexpected roles of the choroid plexus-cerebrospinal fluid system in health and disease

Organisers: Fiona Doetsch and Maria K. Lehtinen

Date: 14 – 17 July 2019

Understanding human birth defects in the genomic age

Organisers: Mustafa Khokha, Karen Liu and John Wallingford

Date: 10 – 13 November 2019

All of the above Workshops are being held at Wiston House , West Sussex, UK.

We offer 10 funded places for early-career researchers to attend our Workshops.

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The Company of Biologists Workshop ‘Symbiosis in the microbial world: from ecology to genome evolution’ took place at Wiston House, West Sussex, UK,from 5 to 9 November 2017.

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The Company of Biologists Workshop ‘Thinking beyond the dish:taking in vitro neural differentiation to the next level’ took place at Wiston House, West Sussex, UK, from 4 to 7 February 2018.

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If you have a vision for such a meeting but don’t have the time to arrange the logistics or raise the funding, then please contact us. We are seeking proposals for future Workshops and provide all necessary support.

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Early-career scientists can apply for a funded place at one of our Workshops. This is a great opportunity that could enhance your career in various ways. Watch the video to hear from some of our previous attendees on why they applied and how it has benefited them.

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The Workshop Committee is made up of a selection of our Directors from different scientific fields and includes:

Explore previous Workshop topics through our video interviews, meeting reports and photo galleries. From neural stem cells through obesity and genome engineering to evolution of the human neocortex, mitosis and more.

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